ABSTRACT
Objective To explore the correlation between the genetic dissimilarity and heterozygosity of mates and the patho?genicity of Schistosoma japonicum in the definitive host. Methods By using seven microsatellite loci markers,S. japonicum genotyping of sixteen pairs randomly mated was performed,the genetic dissimilarity and heterozygosity were calculated between the mates,and the correlation between the genetic dissimilarity and heterozygosity of the mates and the pathogenicity of S. japon?icum in the definitive host was evaluated. Results There was a significant correlation between the genetic similarity of S. ja?ponicum mates and the mean number of eggs per worm pair in the liver and intestinal tissue (r = 0.501 6 ,P 0.05;r=0.265 3,P>0.05,respectively)and the average diameter of granuloma in the liver(r=-0.272 7,P>0.05),respec?tively. There was no correlation between the heterozygosity of the mates and all the pathological parameters of S. japonicum in the definitive host(P > 0.05). Conclusions There is the correlation between the genetic dissimilarity of the mates and the pathogenicity of S. japonicum in the definitive host,and the genetic dissimilarity is greater,pathogenicity is weaker. There is no correlation between heterozygosity of the mates and the pathogenicity of S. japonicum in the definitive host.
ABSTRACT
In recent decades a number of authors have argued that allergen exposure is the major primary cause of asthma and that the global increases in mites allergic asthma prevalence are associated with the increases of exposure to mites-allergens.The knowledge of the acaridan and the allergens is important for planning intervention of mites allergic asthma.The research progress in this field was reviewed in the present paper,including the epidemiology,the mechanism and immunotherapy of mites allergic asthma.
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Objective To investigate the breeding situations of acaroid mites in some dwellings in Zhangjiagang city and the influence on the patients with mites allergic asthma.Methods Acaroid mites in the dust from five different positions of rooms of 40 patients with mites allergic asthma and 40 normal control were counted and identified by light microscope.Results The detection rate of acaroid mites in 400 samples was 52.25%(209/400).A total of 2 746 acaroid mites were detected,which belong to 15 species and 7 families.The highest breeding rate and relative abundance were 46.25%(185/209)and 54.26%(1 490/2 746)respectively,which comes from Pyroglyphidae family,the following families were Acaridae(34.5%;23.89%)and Glycyphagidae(19.25%;18.14%).About the breeding densities of acaroid mites,statistical difference between patients and normal control was found(P
ABSTRACT
Objectives To establish method for collecting haemocytes of Oncomelania hupensis and study its morphology and immunological importance. Methods Referring to the method of haemocytes collection from peripheral lymphoid organ, suspension technique was used for collection of haemocytes from snails, which were then Giemsa-stained and observed under microscope. Stained by gentian violet, number of haemocytes was counted and compared with that of conventional squashing method and needling method by ANOVA and Dunnett-t test. Supernatant from freeze thawing haemocytes was applied for the tests of immuno-precipitation, bacteriostasis, and phagocytosis. SDS-PAGE was used to analyze relative molecular mass of protein ingredients. Results Four kinds of haemocytes were found: round cells with filiform filopodia, acidophilic and basophilic round cells both without filiform filopodia, and spindle cells. The average diameter of the 4 type cells was 10.93, 6.13, 6.08, and 11:06?m, and occupied 50%, 30%, 5%, and 15% respectively. The mean of haemocytes received from suspension, squashing and needling methods was 15 000, 6 600 and 300/ml respectively. ANOVA analysis showed F=281.47, P